The Power-Sepô System is a fully automated
liquid chromatography system, capable of automating the purification and
production of biomolecules using chromatography techniques such as ion
exchange, gel filtration and affinity chromatography. The Power-Sepô
System has the unusual flexibility to run laboratory, pilot or full scale
production employing the same basic unit. The Power-Sepô System can be
expanded to control multi-column and multi-sample applications. The
DMS-6000 Data Management Software allows real time plotting and automatic
documentation of purification data.
plotting of UV, pH, Conductivity, and Temperature.
parameters can be changed during the run.
to save UV, pH, Conductivity and Temperature data every 0.5 seconds.
flow rate ranges 0.02ml ~ 1000ml/min.
data can be reviewed and compressed.
from small scale to production scale purification.
PRINCIPLES OF OPERATION
The hardware consists of a
computer unit and a separation unit. The computer unit communicates with
the separation unit through an RS232 interface. The separation unit
consists of the following modules:
- LC Control Module.
- Pump and Pressure
- Valve Module.
- Valve Drive
- UV Detector.
- pH Electrode.
- Temperature Probe.
- Gradient Module.
- Bubble Detector.
The Power-Sepô System uses
Windows based DMS-6000 real time Data Management Software to coordinate
the entire purification process. The system uses a 4 channel graphics
screen for real time plotting of data which is obtained from a UV
detector, pH and conductivity electrodes, and a temprature probe.
The Power-Sepô can capture
and save the purification data every 0.5 second in a data file. The saved
plot data can be reviewed and compressed up to 1000 times. The Power-Sepô
DMS-6000 Software allows you to display, review and print the results of
a run or a compressed summary and to analyze trends. The report feature
allows you to print the results in graph and/or tabloid form with date,
time, title, file name, and the associated purification program.
Graphs and Tables
The purification data; UV,
pH, and conductivity, can be superimposed on one screen. The Power-Sepô
DMS-6000 Software allows you to review saved data from up to four runs on
your display screen at once. The saved data can be printed as a graph or
a table along with the corresponding purification program.
The entire purification protocol, from sample loading and column washing
to elution and fraction collection, can be programmed on a task by task
basis. The Power-Sepô System can be programmed to collect fractions based
on volume, or UV absorbance. The developed protocol can be run
The Power-Sepô DMS-6000 Software Windows based Editor allows you to
program multiple step purification processes in thousands of
configurations. The purification programs can be individually named and
saved. With the DMS editor you can set the following parameters:
Wide Range Of Flows
The Power-Sepô System
allows you to program a range of flow rates from 0.020 to 1000 ml/minutes
with the same basic unit. For large scale purifications, higher flowrate
pumps are available. For details consult FMS.
Variety of Different Pump Heads
The Power-Sepô's modular
pump head design allows you to choose the best size and type of pump head
for your primary process and optional pump heads for other processes. We offer
a variety of different pump head materials including Teflon, ceramic,
stainless steel, kynar and tygon. Pump heads can be manually replaced in
less than a minute.
Works with Organic Solvents
By replacing the pump head,
and valve modules, the same Power-Sepô system can perform applications
that require organic solvents. FMS 's wide range of pumpheads and valve
materials allow you to use any kind of organic and inorganic reagents.
The heart of the Power-Sepô System is FMS's patented calibration process.
It is capable of learning the optimum purification conditions and
reproducing the same purification conditions over and over again.
UV Detector, pH, & Conductivity Electrodes
The Power-Sepô System
monitors the UV absorption, conductivity, temperature, and pH of the
purified material during the run. The data obtained from the detectors is
plotted in a multi-channel plotting screen and can be stored for future
reference or print out.
The columns are constructed from either PTFE, glass or acrylic. They can
be used for both bench top analytical scale and floor mounted large scale
separations. The columns are engineered in a variety of different sizes
for gel filtration, affinity or ion exchange techniques.
Unlike other commercial systems which have been marketed for either
laboratory, pilot, or process scale purification, the Power-Sepô System
is designed to be used from laboratory to production scale purification
with the same basic unit. The modularity of the Power-Sepô allows the
transition from small to large scale purification to be as easy as
replacing the pump and the valve modules. This feature of the Power-Sepô
System saves money and makes the process development and scale-up an
The low pressure linear
binary and ternary gradient module allows the creation and storage of
unlimited numbers of profiles. The gradient module is interfaced to the
Power-Sepô System and controls the gradient valve module for gradient
Power-Sepô System Configurations:
If you are
performing your purifications manually and the high cost of automation
has prevented you from automating your purifications, you will love our System
#1 configuration. This low cost system interfaces to your existing UV,
column and chart recorder and automates your entire purification process.
If you are
involved with analytical scale purification, our System #2 configuration
offers you a complete laboratory scale purification system.
modular system design of the Power-Sepô System allows you to reconfigure
and expand the system to suit all your purification needs.
development and scale-up stages, Power-Sepô System #4 offers you the
DMS-6000 complete documentation and analysis tools package. This software
package assists you in creating an optimized protocol for your
purification application needs grow from laboratory to pilot to process
scale, System #5 can be used in all stages of scale-up. With System #5 it
is possible to run columns ranging from 1 ml to hundreds of liters.
Information please contact FMS at email@example.com